Degradation of carboxymethyl chitosan by several common periodontal pathogens

doi:10.3969/j.issn.2095-4344.2013.34.014

Abstract

Abstract:

BACKGROUND:In recent years, the degradation in vivo of carboxymethyl chitosan has become a research focus in the fields of periodontal tissue engineering and materials science. However, in the literature involving carboxymethyl chitosan enzymatic degradation, there is no report regarding whether common periodontal pathogens could degradate carboxymethyl chitosan.

OBJECTIVE: To study the degradation of carboxymethyl chitosan by several common periodontal pathogens.

METHODS: 1% and 2% carboxymethyl chitosan anaerobic microbiological culture media were prepared. The colonies of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitan and Actinomyces viscosus were picked up streaking onto the carboxymethyl chitosan medium, respectively, which was then incubated in anaerobic culture bag at 37 ℃. If the see-through circle appeared around the colonies, the result was positive, indicating that the bacteria can degradate carboxymethyl chitosan; on the contrary, it was negative. Afterwards, the diameter of the colonies was measured.

RESULTS AND CONCLUSION: It was observed that the colonies of Porphyromonas gingivalis grew well in the 1% carboxymethyl chitosan medium and the see-through circles appeared around the colonies after incubation for 18 days; meanwhile, the medium containing 2% carboxymethyl chitosan inhibited the growth of Porphyromonas gingivalis and the standard morphological colonies did not form well. The see-through circle did not appear around the colonies of Actinobacillus actinomycetemcomitan and Actinomyces viscosus in the two concentrations of carboxymethyl chitosan media and the colonies were dried-up and lost activity after 2-week incubation. The results indicated that the lower concentration of carboxymethyl chitosan could induce Porphyromonas gingivalis to produce chitosanase and degradate carboxymethyl chitosan. Neither Actinobacillus actinomycetemcomitan nor Actinomyces viscosus could degradate carboxymethyl chitosan.

Key words: biomaterials, biomaterials and drug controlled release, membrane biomaterials, carboxymethyl chitosan, periodontal pathogens, Porphyromonas gingivalis, chitosanase, see-through circle, colonies

CLC Number:

R318

Cao Wen-shuai, Deng Jing, Xu Quan-chen, Zhu Yuan-qi . Degradation of carboxymethyl chitosan by several common periodontal pathogens[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6152-6158.

Figures/Tables 5

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